Seasonal ozone response of mature beech trees (Fagus sylvatica) at high altitude in the Bavarian forest (Germany) in comparison with young beech grown in the field and in phytotrons

Mature beech trees (Fagus sylvatica) grown at two different altitudes in the Bavarian forest were compared with young beech trees grown at nearby field sites or in phytotrons for their macroscopic and physiological responses to different ozone (O-3) exposures. Cumulative O-3 exposure expressed as the sum of hourly mean concentrations above the canopy ranged between 100 and 150 mu l l(-1) h, with the vertical O-3 profiles at the higher altitude site bring enhanced by 30%. O-3 profiles at all sites were reduced by up to 20% with increasing depth within and beneath the canopy. The leaf discoloration that developed in the absence of premature leaf loss was similar in the sun foliage of mature and young trees (including plant grown in the phytotron). Injury became apparent at low O-3 exposures, expressed as accumulated hourly means over a threshold of 40 nl l(-1) (AOT40 < 3.5 mu l l(-1) h) at the lower site in both the mature trees and the young beech at the field site, but only occurred when AOT40 values reached 7 mu l l(-1) h at the upper site, and 6 mu l(-1) h in the phytotrons. However, the association between injury and O-3 exposure was improved when cumulative ozone uptake to sun leaves was the ozone index, used with values of about 3 mmol m(-2) resulting in visible injury in both mature and young beech growing in phytotrons. Under high ozone exposure levels of inositol were lowered, whilst concentrations of lignin like materials were enhanced in mature beech. Similar responses were observed in young beech grown in phytotrons. As the sun foliage was affected by only a small and variable extent each year, the seasonal O-3 impact at high altitude did not appear to pose an acute risk to mature belch trees. (C) 2000 Elsevier Science Ltd. All rights reserved.