Journal
PARASITOLOGY
Volume 120, Issue -, Pages 281-287Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182099005521
Keywords
Trypanosoma brucei; IFN-gamma; trypanosome-derived lymphocyte triggering factor (TLTF)
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Bidirectional signalling between Trypanosoma brucei brucei and CD8(+) T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-gamma. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-gamma in a dose-response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-gamma-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.
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