An ultrasensitive competitive binding assay for the detection of toxins affecting protein phosphatases

An ultrasensitive assay Is described for microcystin-LR and other substances (microcystins, nodularin, okadaic acid, calyculin A, tautomycin) which block the active site of protein phosphatases (PP) 1 and 2A. The assay is based on competition between the unknown sample and [I-125]microcystin-YR for binding to the catalytic subunit of PP2A. The PP2A-bound [I-125]microcystin-YR was stable (half-time of dissociation = 1.8 h), allowing non-bound [I-125]microcystin-YR to be removed by Sephadex G-50 size-exclusion chromatography. Compared to current assays based on inhibition of protein phosphatase activity, the present assay was more robust against interference (from fluoride, ATP, histone, and casein), and had an even better sensitivity. The detection limit was below 50 pM (2.5 fmol) for nodularin and microcystin-LR, and below 200 pM (10 fmol) for okadaic acid. The method was used successfully to detect extremely low concentrations of either microcystin or nodularin in drinking water or seawater, and okadaic acid in shellfish extract. (C) 1999 Elsevier Science Ltd. All rights reserved.