Journal
NEUROIMMUNOMODULATION
Volume 7, Issue 2, Pages 99-105Publisher
KARGER
DOI: 10.1159/000026427
Keywords
NF-kappa B; p65; apoptosis; acute inflammation; caffeic acid phenethyl ester
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Funding
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [ZIAHD000618] Funding Source: NIH RePORTER
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [Z01HD000618] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [Z01BC007363, ZIABC007363] Funding Source: NIH RePORTER
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Nuclear factor kappa-B (NF-kappa B) is a heterodimeric transcription factor with a pivotal role in orchestrating immune and inflammatory processes, Inflammatory cytokines and prostanoids activate NF-kappa B, which, in turn, stimulates expression of cytokines, proteases, adhesion molecules and other inflammatory mediators. Caffeic acid phenethyl ester (CAPE) is a compound that modulates nuclear binding of the NF-kappa B p65 subunit (RelA). To determine whether CAPE decreases the viability of cells participating in host defense, we first tested its in vitro effect on a glucocorticoid-sensitive and -resistant cell line of lymphoid origin. CAPE induced apoptotic cell death in a dose-dependent fashion and to a similar extent in both cell lines. Furthermore, a low concentration of CAPE decreased the LD50 of dexamethasone by 3- to 5-fold. Since therapeutic induction of apoptosis of activated inflammatory cells holds the attraction of destroying effector cells safely without secondary tissue damage, we examined the effects of CAPE in a rat model of carrageenin-induced subcutaneous inflammation. Local administration of CAPE resulted in increased leukocyte apoptosis and marked reduction in exudate leukocyte, neutrophil and monocyte concentrations at the inflammatory site. CAPE decreased expression of cytosolic I kappa B alpha and increased nuclear translocation of p65. These findings may suggest that novel anti-inflammatory therapies can be based upon activation of NF-kappa B-mediated transcription of genes curbing the inflammatory response and that CAPE or its analogs hold therapeutic promise. Copyright (C) 2000 S. Karger AG, Basel.
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