Journal
BIOLOGIA PLANTARUM
Volume 44, Issue 4, Pages 603-606Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1013763208472
Keywords
benzylaminopurine; in vitro culture; medicinal plants; plantlets multiplication
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The in vitro studies with Cardiospermum halicacabum indicated that the different explants, i.e cotyledon, hypocotyl, cotyledonary node, leaf, internode and node had the potential to produce calli on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BAP) and napthalene acetic acid (NAA). Calli of different explant origin showed variable growth responses on different BAP concentrations. The shoots were favourably formed from the calli of leaf and cotyledon explants. The maximum number of shoots were produced from calli subcultured on MS + BAP (17.8 muM). The roots were initiated on growth regulator free MS medium.
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