Specific inhibition of cyclooxygenase-2 results in inhibition of proliferation of oral cancer cell lines via suppression of prostaglandin E-2 production

Prostaglandins (PGs) are known to play important roles in the proliferation of various types of cancer cells. PGs are produced by the action of cyclooxygenase (COX) enzymes, and two forms of COX, COX-1 and COX-2, have been described. Previous studies have demonstrated that overexpression COX-2 is associated with colon carcinogenesis, tumor invasion and metastatic potential of colon cancer. In this study, the role of COX-2 on proliferation of squamous cell carcinoma cell lines was investigated, NS-398, a selective COX-2 inhibitor, inhibited proliferation of NA cells, a squamous cell carcinoma cell line that constitutively expresses COX-2 mRNA. NS-398 suppressed the spontaneous production of PGE(2) by NA cells, and the antiproliferative effect of NS-398 was abolished by addition of PGE(2). Similar results were obtained from experiments using COX-2 antisense oligonucleotide. These results suggest that specific inhibition of COX-2 inhibits proliferation of cancer cells expressing COX-2 mRNA via suppression of PGE(2) production.