Journal
MARINE BIOTECHNOLOGY
Volume 3, Issue 1, Pages 1-3Publisher
SPRINGER-VERLAG
DOI: 10.1007/s101260000043
Keywords
barramundi; DNA marker; genetic diversity; broodstock
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Four microsatellites were identified by screening the DNA sequences of Asian seabass (Lares calcarifer) deposited to GenBank. Two markers each are located in the growth hormone gene (GH) and in the insulin-like growth factor II gene (IGF-2), respectively. The markers were characterized by genotyping 34 Asian seabass individuals. All 4 microsatellites showed polymorphism: the number of alleles per locus ranged from 2 to 11 (average, 5.0), while the expected heterozygosity ranged from 0.51 to 0.85 (average, 0.63) at the 4 loci. Cross-priming with all 4 primer pairs was tested in species belonging to 5 different genera, but no bands were amplified. These microsatellites are the first genomic DNA markers characterized in L. calcarifer thus they may be valuable for research and aquaculture production of this species.
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