Journal
CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE
Volume 4, Issue 1, Pages 51-56Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00075197-200101000-00010
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- NIAMS NIH HHS [R01 AR044474, R01 AR44474] Funding Source: Medline
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR044474] Funding Source: NIH RePORTER
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The most common methods for measuring the incorporation of tracer amino acids into tissue protein are the constant tracer infusion and the flooding dose. The flooding dose is an attractive method for measuring tissue protein synthesis because of its convenience and precision. A primary assumption of the method, that the free amino acid precursor pools are equilibrated with the true precursor pool, aminoacyl-transfer RNA, has recently been validated. When short labelling periods are involved, the large dose of amino acid does not appear to alter protein synthesis. The constant tracer infusion is a satisfactory method from a theoretical point of view, but its use requires the measurement of the protein synthetic precursor pool. The best estimate of the aminoacyl-tRNA precursor pool for the constant infusion method appears to be the acid-soluble tissue pool in muscle and VLDL apolipoprotein B-100 in the liver. The experimental approach chosen for measuring tissue protein synthesis should be dictated by the question being addressed. (C) 2001 Lippincott Williams Wilkins.
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