Journal
MICROBIOLOGICAL RESEARCH
Volume 156, Issue 4, Pages 311-315Publisher
URBAN & FISCHER VERLAG
DOI: 10.1078/0944-5013-00111
Keywords
perchlorate reduction; nitrate reduction; perclace; groundwater
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The perchlorate (ClO4-)-respiring organism, strain perclace, can grow using nitrate (NO3-) as a terminal electron acceptor. In resting cell suspensions, NO3- grown cells reduced ClO4-, and ClO4- grown cells reduced NO3-. Activity assays showed that nitrate reductase (NR) activity was 1.31 mu mol min(-1) (mg protein)(-1) in ClO4- grown cells, and perchlorate reductase (PR) activity was 4.24 mu mol min(-1) (mg protein)(-1) in NO3- grown cells. PR activity was detected within the periplasmic space, with activities as high as 14 mu mol min(-1) (mg protein)(-1). The NR had a pH optimum of 9.0 while the PR had an optimum of 8.0. This study suggests that separate terminal reductases are present in strain perclace to reduce NO3- and ClO4-.
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