Journal
CHEMCATCHEM
Volume 6, Issue 4, Pages 1096-1102Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cctc.201300892
Keywords
aldehydes; cyanides; enzyme catalysis; gels; immobilization
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Funding
- Fundacion Alfonso Martin Escudero (Spain)
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Hydroxynitrile lyase from cassava, Manihot esculenta, (MeHNL) catalyzes the formation of (S)-cyanohydrins from HCN and aldehydes or ketones. Four differently immobilized MeHNLs were prepared: by noncovalent immobilization (celite R-633), covalent immobilization (cross-linked enzyme aggregates, CLEA), encapsulation [in a poly(vinyl alcohol) hydrogel Lentikats] and a combination of the above, an unusual immobilization, CLEA encapsulated in a poly(vinyl alcohol) hydrogel. A comparative study of the recyclability of each immobilized MeHNL was performed. Particular attention was paid to the stability and activity of the new immobilized MeHNL-CLEA-Lentikats and to the minimum enzyme loading required to achieve high yields and enantiomeric excesses. MeHNL-CLEA stability was slightly improved by encapsulation into Lentikats and good recyclability rates at low enzyme loading were obtained. However, MeHNL immobilized on celite R-633 exhibited the best recyclability, giving >95% conversion and an enantiomeric excess of 99% during 12cycles.
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