Journal
BRAZILIAN JOURNAL OF MICROBIOLOGY
Volume 32, Issue 1, Pages 56-60Publisher
SOC BRASILEIRA MICROBIOLOGIA
DOI: 10.1590/S1517-83822001000100013
Keywords
mycovirus; double-stranded RNA; virus-like particles; Aspergillus flavus
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Two stains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication.
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