Journal
JOURNAL OF FORENSIC SCIENCES
Volume 61, Issue -, Pages S241-S245Publisher
WILEY
DOI: 10.1111/1556-4029.12928
Keywords
forensic science; cardiac troponin-T; proteolysis; degradation; postmortem interval
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Funding
- University Grants Commission U.G.C. [17/7-2011(SA-1)]
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Estimation of time of death is an indispensible requirement of every medico-legal autopsy, but unfortunately, there is not a single method by which it could be determined accurately. This study focused on the temperature-dependent postmortem degradation of cardiac troponin-T and its association with postmortem interval (PMI) in human. The analysis involved extraction of the protein, separation by denaturing gel electrophoresis (SDS-PAGE), and visualization by Western blot using cTnT-specific monoclonal antibodies. The area of the bands within a lane was quantified by scanning and digitizing the image using Gel Doc (Universal Hood). The results indicate a characteristic banding pattern among human cadavers (n = 6) and a pseudo-linear relationship between percentage of cTnT degradation and the log of the time since death (r > 0.95), which can be used to estimate the postmortem interval. The data presented demonstrate that this technique can provide an extended time range during which PMI can be more accurately estimated.
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