Journal
CHEMCATCHEM
Volume 2, Issue 1, Pages 73-77Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cctc.200900220
Keywords
amines; asymmetric synthesis; biocatalysis; kinetic resolution; transamination
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Funding
- FFG
- Province of Styria
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Various recombinant (omega-transaminases, overexpressed in E. coli cells and employed as whole-cell catalysts, are tested for the synthesis of enantiomerically pure amines from the corresponding prochiral ketones. Optically pure (S)-amines are obtained by formal reductive amination, consuming just ammonia and a cheap reducing agent (formate) with up to 99% ee and 97% yield. The other enantiomer was accessible by employing the same omega-transaminases in a kinetic resolution starting from racemic amines. A omega-transaminase derived from an Arthrobacter species displayed the highest stereoselectivity for all substrates tested, both for the kinetic resolution of rac-amines and for the amination of ketones.
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