Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 19, Issue 1, Pages 1-19Publisher
HARWOOD ACAD PUBL GMBH
DOI: 10.3109/10242420109103513
Keywords
kinetic resolution; enantioselectivity; diffusion limitation; particle size; immobilized enzyme; Candida antarctica lipase B
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Diffusion limitation in immobilized enzyme particles may decrease the performance of kinetic resolution processes. In this paper a standard reaction plus diffusion model approach, based on homogeneous enzyme distribution, is used to verify experimentally the influence of the particle diameter on the observed enantioselectivity. This is done for a commercial preparation of immobilized Candida antarctica lipase B in the kinetic resolution of racemic 2-butanol by esterification with vinyl butyrate in heptane. For immobilized enzyme particles with particle diameters ranging from 355 - 710 mum the apparent enantiomeric ratio is constant at E-app = 2.5, while for ground particles with a particle diameter of 1 mum the intrinsic enantiomeric ratio is E=4.4. This clear example of the influence of diffusion limitation on the observed enantioselectivity can not be described quantitatively in a straightforward way, because the enzyme seems to be present as a layer in the carrier particles.
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