Journal
CHEMBIOCHEM
Volume 15, Issue 15, Pages 2255-2258Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201402226
Keywords
HNA; mutagenesis; polymerization; universal bases; unnatural bases; XNA
Funding
- FWO (Vlaanderen) [G.078014N]
- Commission of the European Communities (NEST) [029092-Orthosome]
- European Research Council under the European Union/ERC [ERC-2012-ADG_20120216/320683]
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The templating potential of anhydrohexitol oligonucleotides bearing ambiguous bases was studied in vivo, by using a selection screen for mosaic heteroduplex plasmids in Escherichia coli. 1,5-Anhydro-2,3-dideoxy-2-(5-nitroindazol-1-yl)-D-arabino-hexitol showed the greatest ambiguity among the three nucleosides tested. At most two successive ambiguous bases could be tolerated on hexitol templates read in bacterial cells. Hexitol nucleosides bearing simplified heterocycles thus stand as promising monomers for generating random DNA sequences in vivo from defined synthetic oligonucleotides.
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