Journal
CHEMBIOCHEM
Volume 15, Issue 11, Pages 1620-1625Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201402058
Keywords
biomarkers; exosomes; glycosylation; microarrays; urine
Funding
- Spanish Ministry of Science and Innovation (MICINN) [PS09/00526, PI12-01604, CTQ2008-04444/BQA]
- ISCIII-Subdireccion General de Evaluacion and the European Fund for Regional Development (Feder)
- Program Ramon y Cajal of MICINN
- Basque Government [GV PI2012-45]
- Spanish ISCIII-MICINN
Ask authors/readers for more resources
As cellular-derived vesicles largely maintain the biomolecule composition of their original tissue, exosomes, which are found in nearly all body fluids, have enormous potential as clinical disease markers. A major bottleneck in the development of exosome-based diagnostic assays is the challenging purification of these vesicles; this requires time-consuming and instrument-based procedures. We employed lectin arrays to identify potential lectins as probes for affinity-based isolation of exosomes from the urinary matrix. We found three lectins that showed specific interactions to vesicles and no (or only residual) interaction with matrix proteins. Based on these findings a bead-based method for lectin-based isolation of exosomes from urine was developed as a sample preparation step for exosome-based biomarker research.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available