Journal
CHEMBIOCHEM
Volume 15, Issue 18, Pages 2719-2728Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201402441
Keywords
biocatalysis; cytochromes; peptide biosynthesis; secondary metabolism; teicoplanin
Funding
- Deutsche Forschungsgemeinschaft (Emmy-Noether Program) [CR 392/1-1]
- Deutsche Akademischer Austausch Dienst (Group of Eight Australia-Germany Joint Research Co-operation Scheme) [56265933]
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Bacterial cytochrome P450s form a remarkable clade of the P450 superfamily of oxidative hemoproteins, and are often involved in the biosynthesis of complex natural products. Those in a subgroup known as Oxy enzymes play a crucial role in the biosynthesis of glycopeptide antibiotics, including vancomycin and teicoplanin. The Oxy enzymes catalyze crosslinking of aromatic residues in the non-ribosomal antibiotic precursor peptide while it remains bound to the non-ribosomal peptide synthetase (NRPS); this crosslinking secures the three-dimensional structure of the glycopeptide, crucial for antibiotic activity. We have characterized OxyB(tei), the first of the Oxy enzymes in teicoplanin biosynthesis. Our results reveal that OxyB(tei) possesses a structure similar to those of other Oxy proteins and is active in crosslinking NRPS-bound peptide substrates. However, OxyB(tei) displays a significantly altered activity spectrum against peptide substrates compared to its well-studied vancomycin homologue.
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