Journal
CHEMBIOCHEM
Volume 15, Issue 7, Pages 939-945Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201300537
Keywords
antibodies; carbohydrates; glycopeptides; glycosylation; vaccines
Funding
- Leibniz institute for Analytical Sciences (ISAS)
- Fonds der Chemischen Industrie [184/01]
- Deutsche Forschungs Gemeinschaft [WE 4751/2-1]
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In spite of the clear importance of protein O-mannosylation in brain glycobiology, tools are lacking for specific detection, enrichment, and identification of proteins containing these modifycations. We envisioned inducing antibodies that specifically recognize O-mannose glycans on proteins and peptides. With this in mind, we prepared a glycopeptide vaccine construct containing the N-acetyllactosamine-extended mannose motif Gal1-4GlcNAc1-2ManThr, found as a common core structure on almost all mammalian O-mannosyl glycoproteins identified. O-mannose glycosylated amino acid building blocks and the corresponding glycopeptides were prepared by chemical synthesis and then conjugated to an immune carrier protein. After administration of the synthetic vaccine into rabbits, strong immune responses were obtained. Further evaluation by ELISA neutralization experiments and glycopeptide microarrays showed that the induced antibodies were highly specific to the glycopeptide antigen.
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