Journal
CHEMBIOCHEM
Volume 15, Issue 6, Pages 826-828Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201300602
Keywords
absolute configuration; D-amino acids; deuterium exchange; mass spectrometry; peptides; structure elucidation
Funding
- BMBF within the Biokatalyse program
- European Research Council [311477]
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An E. coli strain with deletions in five transaminases (aspC ilvE tyrB avtA ybfQ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide xenotetrapeptide from Xenorhabdus nematophila, by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D-amino acids in the produced peptide was assigned following simple cultivation of the expression strain in D2O.
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