Journal
CHEMBIOCHEM
Volume 13, Issue 8, Pages 1206-1213Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201100747
Keywords
aptamers; fluorescent probes; malachite green; RNA visualization; two-photon absorption
Funding
- ANR [ANR-08-BLAN-0244-02]
- Agence Nationale de la Recherche (ANR) [ANR-08-BLAN-0244] Funding Source: Agence Nationale de la Recherche (ANR)
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The design, preparation and characterisation of a library of malachite green (MG) derivatives for two-photon RNA labelling is described. Some of these MG derivatives exhibit an increased affinity for an MG-aptamer, as well as improved two-photon sensitivity when compared to the classical malachite green chloride. The underlying mechanisms and potential benefits for in vivo RNA visualisation are discussed.
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