Journal
CHEMBIOCHEM
Volume 13, Issue 8, Pages 1161-1166Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201100811
Keywords
cytochromes; directed evolution; hydroxylation; mutagenesis; regioselectivity; steroids
Funding
- Vietnamese Ministry of Education and Training (MOET)
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CYP106A2 is known as a 15 beta-hydroxylase, but also shows minor 11a-hydroxylase activity for progesterone. 11a-Hydroxyprogesterone is an important pharmaceutical compound with anti-androgenic and blood-pressure-regulating activity. This work therefore focused on directing the regioselectivity of the enzyme towards hydroxylation at position 11 in the C ring of the steroid through a combination of saturation mutagenesis and rational site-directed mutagenesis. With the aid of data from a homology model of CYP106A2 containing docked progesterone, together with site-directed mutagenesis of active-site residues (Lisurek et al. ChemBioChem 2008, 9, 14391449), a saturation mutagenesis library at positions A395 and G397 was created. Screening of the library identified the mutants A395I and A395W/G397K as having 11a-hydroxylase activities 8.9 and 11.5 times higher than that of the wild type (WT). In the next step, additional mutations were integrated by a rational site-directed mutagenesis approach to increase the catalytic efficiency. Of the 40 candidates analyzed, the mutants A106T/A395I, A106T/A395I/R409L, and T89N/A395I turned out to display increased 11a-hydroxylase selectivities and activities relative to the WT (14.3-, 12.6-, and 11.8-fold increases in selectivity and 39.3-, 108-, and 24.4- in kcat/Km). In the last step of the study, the best mutants were applied in a whole-cell biotransformation. In these experiments the production (percentage) of 15 beta-hydroxyprogesterone decreased from 50.4?% (wild type) to 4.8?% (mutant T89N/A395I), whereas that of 11a-hydroxyprogesterone increased from 27.7 to 80.9?%, thus demonstrating an impressive regioselectivity.
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