Journal
CHEMBIOCHEM
Volume 13, Issue 13, Pages 1905-1912Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201200396
Keywords
DNA recognition; four-way junctions; FRET; quadruplex DNA; three-way junctions
Funding
- Centre National de la Recherche Scientifique (CNRS)
- Universite de Bourgogne (uB)
- Conseil Regional de Bourgogne (CRB)
- Agence Nationale de la Recherche (ANR) [ANR-10-JCJC-0709, PARI-SSTIC 6, 92-01]
- Agence Nationale de la Recherche (ANR) [ANR-10-JCJC-0709] Funding Source: Agence Nationale de la Recherche (ANR)
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The targeting of higher-order DNA structures has been thoroughly developed with G-quadruplex DNA but not with other structures like branched DNA (also known as DNA junctions). Because these alternative higher-order DNA architectures might be of high biological relevance, we implemented a high-throughput version of the FRET melting assay that enabled us to map the interactions of a candidate with four different DNA structures (duplex- and quadruplex DNA, three- and four-way junctions) in a rapid and reliable manner. We also introduce a novel index, the BONDS (branched and other noncanonical DNA selectivity) index, to conveniently quantify this differential affinity.
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