Journal
CHEMBIOCHEM
Volume 12, Issue 16, Pages 2441-2447Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201100469
Keywords
bioconjugation; cell targeting; epidermal growth factor; multivalency; nanoparticles; viruses
Funding
- National Institutes of Health [CA112075, RR021886, R21EB005364]
- NSF
- [K99EB011530]
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Structurally uniform protein nanoparticles derived from the self-assembly of viral capsid proteins are attractive platforms for the multivalent display of cell-targeting motifs for use in nanomedicine. Virus-based nanoparticles are of particular interest because the scaffold can be manipulated both genetically and chemically to simultaneously display targeting groups and carry a functional payload. Here, we displayed the human epidermal growth factor (EGF) on the exterior surface of bacteriophage Q beta as a C-terminal genetic fusion to the Q beta capsid protein. The co-assembly of wild-type Q beta and EGF-modified subunits resulted in structurally homogeneous nanoparticles displaying between 5 and 12 copies of EGF on their exterior surface. The particles were found to be amenable to bioconjugation by standard methods as well as the high-fidelity copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC). Such chemical derivatization did not impair the ability of the particles to specifically interact with the EGF receptor. Additionally, the particle-displayed EGF remained biologically active promoting autophosphorylation of the EGF receptor and apoptosis of A431 cells. These results suggest that hybrid Q beta-EGF nanoparticles could be useful vehicles for targeted delivery of imaging and/or therapeutic agents.
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