Journal
BLOOD
Volume 97, Issue 1, Pages 324-326Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood.V97.1.324
Keywords
-
Categories
Ask authors/readers for more resources
Reporter mouse strains are important tools for monitoring Cre recombinase-mediated excision in vivo. In practice, excision may be incomplete in a given population due to threshold level or variegated expression of Cre, Hence, it is desirable in many experimental contexts to isolate cells that have undergone excision to assess the consequences of gene ablation, To generate alternative reporter mice, an enhanced green fluorescent protein (EGFP) gene was targeted to the retroviral-trapped ROSA26 locus, Upon Cre-mediated excision of Stop sequences, EGFP was expressed ubiquitously during embryogenesis and in adult tissues (including T cells, B cells, and myeloid cells). Using this new reporter strain, separation of excised from nonexcised cells in vitro was achieved in thymocytes in a noninvasive manner based on activated EGFP expression. This new EGFP reporter strain should facilitate a variety of conditional gene-targeting experiments, including the functional studies of hematopoietic cells in lineage-specific knockout mice. (C) 2001 by The American Society of Hematology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available