Journal
CHEMBIOCHEM
Volume 11, Issue 11, Pages 1563-1573Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201000020
Keywords
carbohydrate chemistry; carbohydrate microarrays; carbohydrates; galectins; glycan binding; lectins
Funding
- Max-Planck Society
- Swiss National Fond (SNF)
- Swiss Federal Institute of Technology (ETH) Zurich
- Alexander von Humboldt Foundation
- Deutsche Forschungsgemeinschaft
- Roche Research Foundation
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Galectins are a class of carbohydrate-binding proteins named for their galactose-binding preference and are involved in a host of processes ranging from homeostasis of organisms to immune responses. As a first step towards correlating the carbohydrate-binding preferences of the different galectins with their biological functions, we determined carbohydrate recognition fine-specificities of galectins with the aid of carbohydrate microarrays. A focused set of oligosaccharides considered relevant to galectins was prepared by chemical synthesis. Structure-activity relationships for galectin-sugar interactions were determined, and these helped in the establishment of redundant and specific galectin actions by comparison of binding preferences. Distinct glycosylations on the basic lactosyl motifs proved to be key to galectin binding regulation-and therefore galectin action-as either high-affinity ligands are produced or binding is blocked. High-affinity ligands such as the blood group antigens that presumably mediate particular functions were identified.
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