Journal
JOURNAL OF IMMUNOLOGY
Volume 166, Issue 1, Pages 498-505Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.166.1.498
Keywords
-
Categories
Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R44AI036810, R01AI025038, R43AI036810] Funding Source: NIH RePORTER
- FIC NIH HHS [TW-00428] Funding Source: Medline
- NIAID NIH HHS [AI-36810, AI-25038] Funding Source: Medline
Ask authors/readers for more resources
Persistent immunity against Leishmania infections in humans is mediated predominantly by CD4(+) T cells of the Th1 phenotype, Herein we report the expression cloning of eight Leishmania Ags using parasite-specific T cell lines derived from an immune donor. The Ags identified by this technique include the flagellar proteins alpha- and beta -tubulin, histone H2b, ribosomal protein S4, malate dehydrogenase, and elongation factor 2, as well as two novel parasite proteins. None of these proteins have been previously reported as T cell-stimulating Ags from Leishmania, beta -tubulin-specific T cell clones generated against Leishmania major amastigotes responded to Leishmania-infected macrophages and dendritic cells, IFN-gamma enzyme-linked immunospot analysis demonstrated the presence of T cells specific for several of these Ags in PBMC from self-healing cutaneous leishmaniasis patients infected with either Leishmania tropica or L. major. The responses elicited by Leishmania histone H2b were particularly striking in terms of frequency of histone-specific T cells in PBMC (1 T cell of 6000 PBMC) as well as the percentage of responding donors (86%, 6 of 7), Ags identified by T cells from immune donors might constitute potential vaccine candidates for leishmaniasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available