Journal
CHEMBIOCHEM
Volume 11, Issue 13, Pages 1896-1904Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201000340
Keywords
carbohydrates; click chemistry; dendrimers; inhibitors; microarrays
Funding
- Dutch Technology Foundation STW
- NWO
- Ministry of Economic Affairs
- Deutsche Forschungsgemeinschaft [FOR 434]
- Konstanz Research School Chemical Biology
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Multivalency is an important phenomenon in protein-carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins.
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