Journal
CHEMBIOCHEM
Volume 10, Issue 4, Pages 735-740Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200800719
Keywords
aggregation; biomineralization; calciprotein particles; fetuin-A; small-angle X-ray scattering (SAXS)
Funding
- German Research Foundation (Deutsche Forschungsgemeinschaft
- Marie Curie Research and Training Network
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We report on the earliest stages of the formation of complexes of calcium phosphate in the presence of the serum protein alpha(2)-HS glycoprotein/fetuin-A termed calciprotein particles (CPPs). Time-resolved small-angle X-ray scattering (TR-SAXS) and stopped-flow analysis were used to monitor the growth of protein mineral particles nucleating from supersaturated salt solutions. It was found that fetuin-A did not influence the formation of mineral nuclei. However, fetuin-A did prevent the aggregation of nuclei and thus mineral precipitation. Hence, fetuin-A shielded spontaneously formed mineral nuclei, leading to stable calciprotein particles in the first stage of mineralization. Fetuin-A is therefore critically required during the earliest stages of the formation of protein-mineral complexes in order to prevent uncontrolled mineralization.
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