Journal
CHEMBIOCHEM
Volume 9, Issue 3, Pages 439-446Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200700536
Keywords
high-throughput screening; in vitro protein expression; microfluidic devices; microreactors; monoclonal droplets
Funding
- Engineering and Physical Sciences Research Council [EP/C013174/1, EP/D048664/1] Funding Source: researchfish
- EPSRC [EP/D048664/1, EP/C013174/1] Funding Source: UKRI
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Microdroplets have great potential for high-throughput biochemical screening. We report the design of an integrated microfluidic device for droplet formation, incubation and screening. Picolitre water-in-oil droplets can be stored in a reservoir that contains 106 droplets. In this reservoir droplets are stable for at least 6 h, which gives an extended timescale for biochemical experiments. We demonstrate the utility of the system by following the in vitro expression of green fluorescent protein. The high efficiency allows protein expression from a single molecule of DNA template, creating monoclonal droplets in which genotype and phenotype are combined in one emulsion compartment.
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