Journal
CHEMBIOCHEM
Volume 9, Issue 8, Pages 1229-1234Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200800062
Keywords
DNA ligands; DNA recognition; DNA; FRET; nucleic acids
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DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligonds that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that mocrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.
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