Journal
JOURNAL OF BACTERIOLOGY
Volume 183, Issue 2, Pages 716-724Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.183.2.716-724.2001
Keywords
-
Categories
Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM060329] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM060329, GM 60329] Funding Source: Medline
Ask authors/readers for more resources
The hyperthermophilic archaeon Pyrococcus furiosus grows optimally at 100 degreesC by the fermentation of peptides and carbohydrates. Growth of the organism was examined in media containing either maltose, peptides (hydrolyzed casein), or both as the carbon source(s), each with and without elemental sulfur (S-0). Growth rates were highest on media containing peptides and S-0, with or without maltose. Growth did not occur on the peptide medium without S-0. S-0 had no effect on growth rates in the maltose medium in the absence of peptides. Phenylacetate production rates (from phenylalanine fermentation) from cells grown in the peptide medium containing S-0 with or without maltose were the same, suggesting that S-0 is required for peptide utilization. The activities of 14 of 21 enzymes involved in or related to the fermentation pathways of P. furiosus were shown to be regulated under the five different growth conditions studied. The presence of S-0 in the growth media resulted in decreases in specific activities of two cytoplasmic hydrogenases (I and II) and of a membrane-bound hydrogenase, each by an order of magnitude. The primary S-0-reducing enzyme in this organism and the mechanism of the S-0 dependence of peptide metabolism are not known. This study provides the first evidence for a highly regulated fermentation-based metabolism in P. furiosus and a significant regulatory role for elemental sulfur or its metabolites.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available