Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 76, Issue 1, Pages 86-90Publisher
WILEY-BLACKWELL
DOI: 10.1002/bit.1029
Keywords
chlorophyllide; polyethylene-glycol (PEG); photosensitizer; photosynthesis; glutamate
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Chlorophyllide a was coupled with alpha-(3-aminopropyl)-omega -methoxypoly(oxyethylene) (PEG-NH2) to form a PEG-chlorophyllide conjugate through an acid-amide bond. The conjugate catalyzed the reduction of methylviologen in the presence of 2-mercaptoethanol. It also catalyzed the photoreduction of NADP(+) or NAD(+) in the presence of ascorbate as an electron donor and ferredoxin-NADP(+) reductase as the coupling enzyme. Utilizing the reducing power of NADPH generated by PEG-chlorophyllide conjugate under illumination, glutamate was synthesized from 2-oxoglutarate and NH4+ in the presence of glutamate dehydrogenase. PEG-chlorophyllide conjugate was quite stable toward light illumination compared with chlorophyll a. The increase in the molecular weight of PEG in the PEG-chlorophyllide conjugates was accompanied by the enhancement of photostability of the conjugate and also by the increased solubility in the aqueous solution. (C) 2001 John Wiley & Sons, Inc.
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