Journal
NEURON
Volume 29, Issue 1, Pages 131-143Publisher
CELL PRESS
DOI: 10.1016/S0896-6273(01)00185-4
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Funding
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS039471] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P01AG006569] Funding Source: NIH RePORTER
- NIA NIH HHS [P01 AG06569] Funding Source: Medline
- NINDS NIH HHS [R01 NS39471] Funding Source: Medline
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The active zone is a specialized region of the presynaptic plasma membrane where synaptic vesicles dock and fuse. In this study, we have investigated the cellular mechanism underlying the transport and recruitment of the active zone protein Piccolo into nascent synapses. Our results show that Piccolo is transported to nascent synapses on an similar to 80 nm dense core granulated vesicle together with other constituents of the active zone, including Bassoon, Syntaxin, SNAP-25, and N-cadherin, as well as chromogranin B. Components of synaptic vesicles, such as VAMP 2/synaptobrevin II, synaptophysin, synaptotagmin, or proteins of the perisynaptic plasma membrane such as GABA transporter 1 (GAT1), were not present. These studies demonstrate that the presynaptic active zone is formed in part by the fusion of an active zone precursor vesicle with the presynaptic plasma membrane.
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