Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 82, Issue 2, Pages 187-199Publisher
WILEY
DOI: 10.1002/jcb.1145
Keywords
bone marrow stromal cells; BMP-7/OP-1; cell differentiation; adipocyte; osteoblast
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Funding
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK046942] Funding Source: NIH RePORTER
- NIDDK NIH HHS [DK46942] Funding Source: Medline
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We studied the effects of BMP-7/OP-1 on growth and differentiation of bone marrow stromal cells. BMS2, a mouse bone marrow stromal cell line capable of differentiating into adipocytes and osteoblasts, were treated in a serum-free medium containing differentiation agents that favor the expression of both lineages. BMP-7/ OP-1 stimulated cell proliferation and differentiation concomitantly. These effects were dose- and growth phase-dependent. Cells were more sensitive to the treatment early in the culture (30-40% confluence) with a significant increase in cell proliferation and markers of differentiation at low concentrations. When treated later in the growth phase (90-100% confluence), no significant increase in cell proliferation was seen. The concentration requirement for cells later in the culture to reach an equivalent degree of differentiation was 3-10- fold higher than for cells treated early. In both cases, the effects on adipocyte differentiation were biphasic; low concentrations stimulated adipocyte differentiation which was inhibited at higher concentrations where stimulation of osteoblast markers were observed. We conclude that cell proliferation and cell differentiation into adipocyte/osteoblast can occur simultaneously under BMP-7/OP-1 treatment. J. Cell. Biochem. 82: 187-199, 2001. dagger Published 2001 Wiley-Liss, Inc.
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