Journal
CHANNELS
Volume 8, Issue 5, Pages 452-457Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/19336950.2014.949162
Keywords
Brefeldin A; Dynasore; LC1; MAP1B; N-type channels; trafficking; UBE2L3
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Funding
- Conacyt [128707-Q, IN-205914]
- PAPIIT-UNAM (Mexico)
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We reported recently a new mechanism by which the neuronal N-type Ca2+ (Ca(V)2.2) channel expression may be regulated by ubiquitination. This mechanism involves the interaction between the channel and the light chain (LC1) of the microtubule associated protein B (MAP1B). We also showed that MAP1B-LC1 could interact with the ubiquitin-conjugating E2 enzyme UBE2L3 and that the ubiquitination/degradation mechanism triggered by MAP1B-LC1 could be prevented by inhibiting the ubiquitin-proteasome proteolytic pathway. We now report that MAP1B-LC1 can interact with the 2 main variants of the Ca(V)2.2 channels (Ca(V)2.2e37a and Ca(V)2.2e37b) and that the MAP1B-LC1-mediated regulation most likely involves an internalization of the channels via a dynamin and clathrin-dependent pathway. In addition, here we propose that this novel mechanism of Ca-V channel regulation might be conserved among N-type and P/Q-type channels.
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