Journal
CHANNELS
Volume 7, Issue 4, Pages 238-242Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/chan.25350
Keywords
Synechocystis; mechanosensitive channel; hypoosmotic stress adaptation; circadian rhythm
Categories
Funding
- JSPS [22380056, 24246045, 24580100, 24658090, 25292055]
- Grants-in-Aid for Scientific Research [24658090, 24657002, 24580100, 24580135, 23570003, 22380056] Funding Source: KAKEN
Ask authors/readers for more resources
Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that symscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available