Journal
CHANNELS
Volume 6, Issue 2, Pages 86-93Publisher
LANDES BIOSCIENCE
DOI: 10.4161/chan.19946
Keywords
TRP channels; gating mechanism; constitutive activity; membrane lipids
Categories
Funding
- National Eye Institute (NEI) [R01 EY 03529]
- US-Israel Bi-National Science Foundation (BSF)
- Israel Science Foundation (ISF)
- German Israeli Foundation (GIF)
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The Drosophila light activated TRP and TRPL channels have been a model for TRPC channel gating. Several gating mechanisms have been proposed following experiments conducted on photoreceptor and tissue cultured cells. However, conclusive evidence for any mechanism is still lacking. Here, we show that the Drosophila TRPL channel expressed in tissue cultured cells is constitutively active in S2 cells but is silent in HEK cells. Modulations of TRPL channel activity in different expression system by pharmacology or specific enzymes, which change the lipid content of the plasma membrane, resulted in conflicting effects. These findings demonstrate the difficulty in elucidating TRPC gating, as channel behavior is expression system dependent. However, clues on the gating mechanism may arise from understanding how different expression systems affect TRPC channel activation.
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