Journal
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
Volume 20, Issue 4-7, Pages 501-506Publisher
MARCEL DEKKER INC
DOI: 10.1081/NCN-100002325
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We have synthesized more than 30 different deoxyribonucleosides and triphosphates with modifications either in the base or the phosphate moiety as analogs of 2'-dGTP for DNA sequencing applications. All the modified nucleoside triphosphates were tested as substrates for DNA polymerases, including Sequenase(TM) T7 DNA polymerase or Thermo Sequenase(TM) DNA polymerase. Two of the analogs, 7-ethyl-7-deaza-dGTP and 7-hydroxymethyl-7-deaza-dGTP meet our requirements as better sequencing reagents.
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