Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 52, Issue 354, Pages 77-84Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jexbot/52.354.77
Keywords
copper; lipids; plasma membrane; superoxide; Triticum durum
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The effects of in vivo copper on the lipid composition of root plasma membrane and the activities of membrane-bound enzymes, such as NADPH-dependent oxidases and lipoxygenase, were studied. Plants were grown in hydroponic culture for 11 d without Cu supply or in the presence of 50 muM Cu. Control plants were supplied with 0.3 muM Cu. Growth of roots was severely affected in the 50 muM Cu-grown plants, whereas roots grown in Cu-deficient solution did not show any difference in comparison with the control. The 50 muM Cu concentration caused an increase in the leakage of K+ ions as well, Excess metal supply resulted in a decrease in the total lipid content of plasma membrane, a higher phospholipid amount and a reduction of steryl lipids (free sterols, steryl glycosides and acylated steryl glycosides), Cu depletion in the growth solution had only a slight effect on the plasma membrane lipid composition, In comparison with the control, only the excess of Cu caused a decrease in the lipid to protein ratio as well as a change in the phospholipid composition, with a lower phosphatidylcholine to phosphatidylethanolamine ratio. The degree of unsaturation of root plasma membranes decreased following the 0 Cu treatment and even more after the 50 muM Cu supply, Plasma membranes of wheat grown under metal deficiency and excess showed increased NADPH-dependent superoxide-producing oxidase activities, whereas membrane-bound lipoxygenase was not increased or activated due to Cu treatments, The consequences of changes in plasma membrane lipid composition and activated oxygen production as a result of Cu treatments are discussed.
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