Chemiluminescence flow sensor for hydrazine with immobilized reagents

A novel chemiluminescence (CL) sensing system for hydrazine combined with flow-injection technique is presented in this paper. The analytical reagents, luminol and hexacyanoferrate(III), were both immobilized on an anion-exchange column. The CL signal produced by the reaction between luminol and hexacyanoferrate(III), which were eluted from the column through sodium phosphate injection, was decreased in the presence of hydrazine. The decrease in CL intensity is linear with hydrazine concentration in the range from 0.1 to 100.0 ng(.)ml(-1); and the detection limit was 0.04 ng(.)ml(-1) (3 sigma). A complete analysis could be performed in 2 min with a relative standard deviation of less than 5.0%. The flow sensor was stable for over 400 analyses and was applied successfully to the determination of hydrazine in artificial samples.