A comparative study of the effect of pH and inorganic carbon resources on the photosynthesis of three floating macroalgae species of a Mediterranean coastal lagoon

This study examines the effect of pH changes on photosynthetic characteristics and the role of dissolved inorganic carbon (DIC) in determining the dominance of three species of macroalgae Chaetomorpha linum (O.F. Muller) Kutzing, Gracilaria verrucosa (Hudson) Papenfuss and Ulva sp. in a Mediterranean coastal lagoon. Fluctuations of pH were measured in the lagoon in summer. Water column CO, and HCO; concentrations inside the algal mat showed significant diurnal fluctuations, from a morning peak to an afternoon low, decreasing 96 and 40%, respectively. The response of photosynthesis to increased pH. was examined in laboratory conditions in spring (May) and summer (July). The photosynthetic rate declined rapidly at pH above 8.5 and below 6.5. G. verrucosa responded differently in spring and summer showing acclimation to higher pH in summer than in spring. In Ulva sp. incubations, we observed optimum photosynthesis between pH 6 and 7.5. The decrease in photosynthetic rate below pH 6 was lower (12.30%) than above pH 8 (81.03%). This difference may be related to the origin of the macroalgae, suggesting acclimation to the original pH of the environment. Results from instantaneous photosynthesis measurements indicate that low DIC-availability limits the photosynthetic capacity of G. verrucosa,, C. linum and Ulva sp. in spring and of C. linum at high irradiances in summer. Our results also show that G. verrucosa has a higher efficiency at low CO2 concentrations than C. linum and Ulva sp. It is suggested that G. verrucosa may be better adapted to maintain higher photosynthetic rates than other macroalgae in conditions of tissue N sufficiency and low water DIC concentrations which are typical of shallow coastal environments in summer. (C) 2001 Elsevier Science B.V. All rights reserved.