Journal
CEREBRAL CORTEX
Volume 25, Issue 10, Pages 3290-3302Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhu125
Keywords
Cerebral cortex; clonal analysis; CLoNe; fate-mapping; intermediate progenitors; subventricular zone
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Funding
- Medical Research Council, UK
- Biotechnology and Biological Sciences Research Council, UK
- German Research Foundation (DFG)
- German Research Foundation (Emmy Noether Programme) [AR 732/1-1]
- Human Frontiers Science Program
- Goodger Scholarship
- Felix Scholarship
- University of Oxford RCUK Access Block Grant
- BBSRC [BB/I021833/1] Funding Source: UKRI
- MRC [G0900901] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I021833/1] Funding Source: researchfish
- Medical Research Council [G0900901] Funding Source: researchfish
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The individual contribution of different progenitor subtypes towards the mature rodent cerebral cortex is not fully understood. Intermediate progenitor cells (IPCs) are key to understanding the regulation of neuronal number during cortical development and evolution, yet their exact contribution is much debated. Intermediate progenitors in the cortical subventricular zone are defined by expression of T-box brain-2 (Tbr2). In this study we demonstrate by using the Tbr2(Cre) mouse line and state-of-the-art cell lineage labeling techniques, that IPC derived cells contribute substantial proportions 67.5% of glutamatergic but not GABAergic or astrocytic cells to all cortical layers including the earliest generated subplate zone. We also describe the laminar dispersion of clonally derived cells from IPCs using a recently described clonal analysis tool (CLoNe) and show that pair-generated cells in different layers cluster closer (142.1 +/- 76.8 mu m) than unrelated cells (294.9 +/- 105.4 mu m). The clonal dispersion from individual Tbr2 positive intermediate progenitors contributes to increasing the cortical surface. Our study also describes extracortical contributions from Tbr2+ progenitors to the lateral olfactory tract and ventromedial hypothalamic nucleus.
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