Journal
CARBOHYDRATE POLYMERS
Volume 44, Issue 1, Pages 41-50Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0144-8617(00)00189-2
Keywords
Sclerotium rolfsii; rheological properties; conformation
Categories
Ask authors/readers for more resources
Scleroglucan produced by Sclerotium rolfsii ATCC 201126 exhibited pseudoplastic behaviour and an exponential relationship between apparent viscosity and polymer concentration. Viscosity showed strong heat-stability (100 degreesC/60 min). Tolerance up to 20% w/v NaCl and over a wide pH range (0-13) was also found. A sharp decrease on viscosity at NaOH concentrations higher than 0.15 M was observed due to dissociation of the triple-helix. According to intrinsic viscosity determinations and gel filtration chromatography on Sepharose CL-4B, the weight-average molecular weight of native polysaccharide was about 5 x 10(6) Da for the tripler and 1.6 x 10(6) Da for the random coil. These values, in addition to the change in complexation with Congo Red, support the idea of the triple-helical conformation in neutral or slightly alkaline solutions (<0.15 M NaOH), and single chains in a highly alkaline environments. (C) 2001 Elsevier Science Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available