Journal
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
Volume 76, Issue 1, Pages 30-34Publisher
HEADLEY BROTHERS LTD
DOI: 10.1080/14620316.2001.11511322
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An efficient protocol for micropropagation of Rosa damascena Mill. has been established using single node segments from mature bushes. The effect of thidiazuron has been compared with that of Nb-benzyladenine (BA) on in vitro shoot proliferation. The cultures initiated on medium supplemented with thidiazuron (TDZ) and/or cultured continuously on TDZ containing medium for 32-48 weeks exhibited considerably more shoot proliferation and growth during subsequent culture on a medium containing BA. Shoots induced on TDZ containing medium and then sub-cultured (8-12 times) on medium containing BA, attained the capacity to grow and proliferate: on a medium free from plant growth regulators (PGR). Microshoots from TDZ induced cultures could be rooted easily on indole-3-butyric acid (IBA) supplemented medium. A short treatment with IBA (100 muM; 12 h) was found to be very effective for root induction. The rooted plants were successfully transferred to pots after 15 d of hardening in a mist chamber with about 70% survival.
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