Journal
CELL DEATH AND DIFFERENTIATION
Volume 8, Issue 1, Pages 38-43Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.cdd.4400800
Keywords
FRET; caspase; GFP; single cell assay
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Most forms of apoptosis involve activation of caspases and it is likely that differences between cells in their ability to activate caspases contributes to the responsiveness of any given cell within a population to apoptotic stimuli. To study the molecular mechanisms that underlie such differences, it is necessary to measure caspase activity in individual cells. Here, we describe a method that allows the continuous monitoring of caspase activity in individual, living mammalian cells. This approach allows studies of the kinetics of caspase activation to be performed in individual cells within a population, We demonstrate that in a group of cells where some cells die and some cells survive in response to the same stimulus, the cells that die can be differentiated from those that survive based on the amount of caspase activity in each cell several hours before death occurs.
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