Journal
CEREBRAL CORTEX
Volume 20, Issue 7, Pages 1756-1767Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhp241
Keywords
HCN currents; neural excitability; orexin A; prelimbic cortex; pyramidal neurons
Categories
Funding
- National Natural Foundation of China [30800450]
- Medicine and Health Scientific Foundation [06MB237]
- National Basic Research Program of China [2006CB500800]
Ask authors/readers for more resources
The hyperpolarization-activated/cyclic nucleotide (HCN)-gated channels make important contributions to neural excitability. In prefrontal cortex, HCN channels are localized on the distal dendrites of layer V pyramidal neurons and decrease neural excitability when they are open. In the present study, using whole-cell voltage clamp recordings, the effect of an arousal peptide, orexin A, on HCN currents in layer V pyramidal neurons from mouse prelimbic cortex (PL), the homolog of the prefrontal cortex was investigated. The results demonstrated that orexin A suppressed HCN currents and shifted their activation curve to a more negative direction. This action of orexin A was blocked by SB334867, an orexin receptor 1 (OXR1) blocker and bisindolylmaleimide, a protein kinase C (PKC) inhibitor, indicating the involvement of OXR1 and PKC. The excitatory effect of orexin A on PL pyramidal neurons was enhanced when HCN currents were diminished, while attenuated when HCN currents were enlarged. In summary, orexin A inhibits HCN currents and enhances excitability of pyramidal neurons in PL, which may contribute to arousal and cognition.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available