Journal
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
Volume 291, Issue 8, Pages 645-648Publisher
URBAN & FISCHER VERLAG
DOI: 10.1078/1438-4221-00180
Keywords
PFGE pattern analysis; DNA degradation; Tris radicals; thiourea
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A number of S. enterica and E. coli strains appeared sensitive to a rapid DNA degradation during the course of PFGE pattern analysis. This kind of DNA degradation could not be stopped by intensive treatment with proteinase K, formalin treatment, or other modifications of the protocol for the isolation of intact chromosomal DNA. However, the application of 100 muM thiourea into the running buffer gave rise to clear-cut PFGE patterns and in turn to an overall typeability.
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