Direct organogenesis from mature leaf and petiole explants of Eryngium foetidum L.

Eryngium foetidum L. plants were regenerated from mature leaf and petiole explants through direct organogenesis without intervening callus phase. From leaf explants, adventitious multiple shoots raised on Murashige and Skoog (MS) medium supplemented with 4.43 muM benzylaminopurine (BAP) and 0.57 muM indole-3-acetic acid (IAA), whereas in petiole explants shoot regeneration occurred at 8,86 muM BAP and 0.57 muM IAA. 80 % of the leaf explants and 44 % of petiole explants produced shoots after four weeks of culture. The regenerated plants were rooted on MS medium supplemented with 2.46 muM indole-3-butyric acid and 2.88 muM gibberellic acid. The plants were successfully established in the soil and showed 70.9 % survival in the field.