Brassinolide effect on growth of apical meristems, ethylene production, and abscisic acid content in potato tubers

Treatment of intact potato (Solanum tuberosum L., cv. Nevskii) tubers with 24-epibrassinolide (EB) resulted in prolonged deep dormancy, increased production of ethylene and higher contents of free and bound abscisic acid (ABA) in buds. EB at the most efficient concentration 0.01 mg dm(-3), applied immediately after tuber harvest, inhibited sprouting by 36 - 38 d, increased ethylene formation after 1 and 7 d of storage by almost 300 and 150 %, respectively, and increased the content of both free and bound ABA during the whole period of storage (on average by about 80 %). Electron microscopic and morphometric studies showed that EB brings about a decrease in cell volume in tunica and all types of meristems and an increase in the number of vacuoles, accompanied by a decrease in their volume.