Journal
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
Volume 38, Issue 1, Pages 1-10Publisher
SPRINGER
DOI: 10.1079/IVP2001243
Keywords
mist; aeroponics; bioreactor; hairy roots; light; artemisinin; productivity
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In vitro cultures are being considered as an alternative to agricultural processes for producing valuable secondary metabolites. Most efforts that use differentiated cultures instead of cell suspension cultures have focused on transformed (hairy) roots. Bioreactors used to culture hairy roots can be roughly divided into three types: liquid-phase, gas-phase, or hybrid reactors that are a combination of both. The growth and productivity of hairy root cultures are reviewed with an emphasis on successful bioreactors and important culture considerations. The latter include strain selection, production of product in relation to growth phase, media composition, the gas regime, use of elicitors, the role of light, and apparent product loss. Together with genetic engineering and process optimization, proper reactor design plays a key role in the development of successful large-scale production of secondary metabolites from plant cultures.
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